IL-1β Secretion in WT vs Atm-/- Macrophages Under Nucleic Acid and LPS+ATP Stimulation #1730148 (License: Personal Use)

This quantitative bar graph displays IL-1β concentrations (pg/ml) measured in supernatants from WT (black bars) and Atm-/- (white bars) macrophages after stimulation with cytosolic DNA analogs (dA:dT, dG:dC, CT-DNA) or LPS+ATP. Error bars indicate standard deviation, revealing consistently heightened IL-1β release in ATM-deficient cells, suggesting loss of ATM-mediated suppression of inflammasome signaling. The strongest response occurs with LPS+ATP, where both genotypes show peak secretion but Atm-/- exceeds WT by ~20%.

Used in immunology or molecular biology research articles, grant reports, or educational resources discussing DNA-sensing pathways, inflammasome regulation, and ATM kinase function in innate immunity; targets researchers and students seeking mechanistic insights into cytokine dysregulation in ATM deficiency (e.g., ataxia-telangiectasia).