DNA Microarray Workflow Explained: Tumor vs Normal DNA Analysis #3510240 (License: Personal Use)

The workflow begins with DNA extraction and digestion, followed by differential fluorescent labeling: tumoral DNA with Cy5 (red) and normal DNA with Cy3 (green). Equal amounts are co-hybridized to a microarray chip containing gene-specific probes. After scanning, image analysis software quantifies fluorescence ratios to detect copy number variations-green spots indicate deletion, red indicate amplification, and yellow show balanced expression. Spotting and probe design ensure high-resolution genomic profiling.

Used in oncology research and molecular diagnostics webpages to explain comparative genomic hybridization (CGH) via microarrays; targets researchers, students, and clinicians seeking to understand cancer genomics techniques.