IPTG-Induced LasI Expression in E. coli Plasmid System #1404166 (License: Personal Use)

This schematic illustrates an inducible gene expression system where IPTG (isopropyl β-D-1-thiogalactopyranoside) inactivates the LacI repressor, allowing RNA polymerase to transcribe the downstream LasI gene under the pLac promoter. The plasmid backbone includes B0034 (a ribosome binding site), and LasI is produced as a quorum-sensing signal synthase. Multiple LasI molecules are shown accumulating post-induction.

Used in educational or research web pages explaining synthetic biology circuits, quorum sensing, or inducible promoters; matches user intent for understanding genetic circuit design and molecular cloning workflows.