DNA Sequencing Chromatogram Explained | Sanger Sequencing Readout #3232171 (License: Personal Use)

This image displays a Sanger sequencing chromatogram, where fluorescently labeled nucleotides generate distinct colored peaks-black for guanine (G), green for adenine (A), red for thymine (T), and blue for cytosine (C). The x-axis represents nucleotide position (e.g., 120-130), and the y-axis reflects signal intensity; the sequence "GAT AAAT CT GG TCTT ATTCC" is called from the peak order. Such chromatograms are essential for validating PCR products, confirming gene edits, or diagnosing mutations.

Used in molecular biology labs and bioinformatics education to illustrate raw sequencing output; commonly appears in protocols, publications, and diagnostic reports where users seek to interpret or validate DNA sequences.