Protein Extraction Protocol Workflow - Step-by-Step Laboratory Procedure #3886974 (License: Personal Use)

This diagram illustrates a standardized protein extraction protocol starting with tissue weighing in a balance, followed by lysis using SDS, Tris, HCl, DTT, and ddH₂O. Bromophenol blue is added as a tracking dye before loading into a microcentrifuge tube, which undergoes heating and vortexing to ensure complete solubilization and denaturation prior to analysis. The final output is a prepared sample ready for electrophoresis or Western blotting.

Used in molecular biology and biochemistry educational resources, lab manuals, or protocol documentation websites; supports users seeking visual guidance on protein sample preparation for assays like SDS-PAGE or immunoblotting.